Research Topics

We utilize Synthetic Biology, Cellular Engineering, and Imaging techniques to study:

Cancer stem cell niche in inflammatory breast cancer

Epithelial and immune cell polarity

Organelle biogenesis and coupling

Dysregulation of intracellular transport

Selected Publications

E.B. Samson, D.S. Tsao, J. Zimak, R. Tyler McLaughlin, N.J. Trenton, E.M. Mace, J.S. Orange, V. Schweikhard, M.R. Diehl (2017).
"The coordinating role of IQGAP1 in the regulation of local, endosome-specific actin networks."
Biology Open, accepted.

Wolfe AR, Trenton NJ, Debeb BG, Larson R, Ruffell B, Chu K, Hittelman W, Diehl M, Reuben JM, Ueno NT, Woodward WA (2016).
"Mesenchymal stem cells and macrophages interact through IL-6 to promote inflammatory breast cancer in pre-clinical models."
Oncotarget. 2016 Oct 15.

AR Wolfe, BG Debeb, L Lacerda, RL Larson MS, A Bambhroliya, X Huang, F Bertucci, P Finetti, D Birnbaum, S Van Laere, P Diagaradjan, B Ruffell, NJ Trenton, K Chu, WN Hittelman, MR Diehl, I Levental, NT Ueno, WA Woodward (2015).
"Simvastatin prevents triple-negative breast cancer metastasis in pre-clinical models through regulation of FOXO3a."
Breast Cancer Research and Treatment, 154(3):495-508.

R. Tyler McLaughlin, MR Diehl, AB Kolomeisky (2015).
"Collective Dynamics of Cytoskeletal Motor Proteins."
Soft Matter, 12, 14-21.

Rogers A, Constantinou PE, Jamison DK, Driver JW, Diehl MR (2014).
"Construction and analyses of elastically coupled multiple-motor systems."
Methods in Enzymology, Volume 540: Chapter 11.

Efremov, A. K., Radhakrishnan, A., Tsao, D. S., Bookwalter, C. S., Trybus, K. M., Diehl, M. R. (2014).
"Delineating cooperative responses of processive motors in living cells."
Proceedings of the National Academy of Sciences,111(3), E334-E343.

Tsao, D. S., & Diehl, M. R. (2014).
"Molecular motors: Myosins move ahead of the pack."
Nature Nanotechnology, 9(1), 9-10.

K. Uppulary, J.W. Driver, M.R. Diehl, A.B. Kolomeisky (2013).
"Analysis of cooperative responses of multiple kinesin motors to structural and chemical factors."
Journal of Cellular and Molecular Bioengineering 6, 38-47.

Diehl, M.R. (2012).
"Templating a Molecular Tug-of-War."
Science 338, 626-627.

Zimak, J., Schweller, R. M., Duose, D. Y., Hittelman, W. N., & Diehl, M. R. (2012).
"Programming in Situ Immunofluorescence Intensities through Interchangeable Reactions of Dynamic DNA Complexes."
ChemBioChem,13(18), 2722-2728.

Schweller, R.M. Zimak, J. Duose, D.Y. Qutub, A.A. Hittelman, W.N. Diehl, M.R. (2012).
"Multiplexed in situ Immunofluorescence via Dynamic DNA Complexes."
Angewandte Chemie International Edition 51, 9292-9296.

Uppulury, K. Efremov, A.K. Driver, J.W. Jamison, D.K. Diehl, M.R. and Kolomeisky, A. (2012).
"How the Interplay Between Mechanical and Non-Mechanical Interactions Affect Multiple Kinesin Dynamics."
The Journal of Physical Chemistry B 116. 8846-8855.

Lu, H. Efremov, A.K. Bookwalter, C.S. Krementsova, E.B. Driver, J.W. Trybus, K.M. Diehl, M.R. (2012).
"Collective Dynamics of Elastically-Coupled Myosin V Motors."
The Journal of Biological Chemistry 287. 27753-27761.

Duose, D.Y. Schweller, R.M. Zimak, J. Rogers, A. Hittelman, W. Diehl, M.R. (2012).
"Configuring Robust DNA Strand Displacement Reactions for In Situ Molecular Analyses."
Nucleic Acids Research 40, 3289-3298.

Jamison, D.K. Driver, J.W. Diehl, M.R. (2011).
"Cooperative Responses of Multiple Kinesins to Variable and Constant Loads."
Journal of Biological Chemistry 287, 3357-3365.

Driver, J.W. Jamison, D.K. Uppulury, K. Rogers, A.R. Kolomeisky, A.B. Diehl, M.R. (2011).
"Productive Cooperation Among Processive Motors Depends Inversely on their Mechanochemical Efficiency."
Biophysical Journal 101, 386-395.

Duose, D.Y. Schweller, R.M. Hittelman, W.N. Diehl, M.R. (2010).
"Multiplexed and Reiterative Fluorescence Labeling via DNA-Circuitry."
Bioconjugate Chemistry 21, 2327-2331.

Jamison, D.K. Driver, J.W. Rogers, A.R. Constantinou, P.E. Diehl, M.R. (2010).
"Two Kinesins Transport Cargo Primarily via the Action of One Motor: Implications for Intracellular Transport."
Biophysical Journal 99, 2967-2977.

Driver, J.W. Rogers, A.R. Jamison, D.K. Das, R.K. Kolomeisky, A.B. Diehl, M.R. (2010).
"Coupling Between Motor Proteins Determines Dynamic Behaviors of Motor Protein Assemblies."
Physical Chemistry Chemical Physics 12, 10398-10405.

Rogers, A.R. Driver, J.W. Constantinou, P.E. Diehl, M.R. (2009).
"Negative Interference Dominates Collective Transport of Kinesin Motors in the Absence of Load."
Physical Chemistry Chemical Physics 11, 4882-4912.

Constantinou, P.E. Diehl, M.R. (2009).
"The Mechanochemistry of Integrated Motor Protein Complexes."
Journal of Biomechanics 43, 31-37.

Schweller, R.M. Constantinou, P.E. Frankel, N.W. Narayan, P. Diehl, M.R. (2008).
"Design of DNA-Conjugated Polypeptide-based Capture Probes for the Anchoring of Proteins to DNA Matrices."
Bioconjugate Chemistry 19, 2304-2307.


Principal Investigator:

Research Assistant Professor:

Volker Schweikhard, PhD

Multi-scale fluorescence microscopy methods to investigate the functional organization of multi-cellular communities and spatiotemporal dynamics of cell state transitions

Graduate Students:

David Tsao

Dynamics of sub-cellular processes

Nicholaus Trenton

Identifying and understanding the cancer stem cell niche in inflammatory breast cancer

Tyler McLaughlin

Quantifying organelle life cycle dynamics with synthetic gene systems